Calculate pH after addition of base?

04 February 2021 2,232 4 View

Western blot with very acidic proteins, any suggestions?

I am trying to do a Western Blot to detect acidic proteins (~30kDa) that have a PI of ~3.5, due to the presence of Asp and Glu repeats. The proteins are about 80% pure. After I ran them on...

24 January 2021 7,226 3 View

How does one create IFORM contours?

Hello: Can anyone assist with plotting IFORM contours. I have been trying to plot contours using Hs and Tp as variables. The marginal distribution is a GPD-Kernel density and the conditional...

12 December 2020 4,355 3 View

How can I transfer heat between groups in Gromacs?

Is it possible to transfer heat from one group to another using simulated aneeling technique in the Gromacs software? I would like to transfer heat from a gold nanoparticle (group 1 from 310 to...

20 November 2020 9,062 3 View

Would ER NiCrMo-3 be the best option for weld overlay of carbon and low alloy steels?

Alloy 625 is the most popular alloy for weld overlay. Without considering the price factor, would NiCrMo-3 be the best technical option for weld overlay of API 5L and low alloy steels, in general,...

24 August 2020 2,478 4 View

Dose-response on non-Michaelian response enzymes, how?

Hello For typical dose-response assays, our lab usually uses steady state intervals for defining the difference between control and tested compound. For those assays, we typically use the angular...

23 July 2020 8,662 2 View

What is the best way to graph an SVM classification in R or Python?

I'm trying to find a way to graph a classification made by the support vector machine algorithm. Something like a PCA or LDA chart would be excellent. Any help will be welcome.

26 April 2020 4,588 1 View

Gene therapy with transgenics viruses Covid-19?

Viral load and symptomatic picture Is there any relationship between the viral load of exposure and contamination, in number of viruses and contamination processes, and the symptomatic picture in...

08 April 2020 6,349 2 View

Is it allowed to publish part of a journal paper(under review) as a conference paper?

Is it allowed to publish part of a journal paper under review as a conference paper? I have submitted a full research paper to a journal. I'm waiting for the review answer and I want to submit...

01 April 2020 430 6 View

Do KG-1a human macrophages need to be activated in some way?

Hello everyone. I was wondering if you can help me clarify this question. I am seeking to perform infection assays with bacteria, using this human cell line. Research articles regarding infection...

24 March 2020 9,884 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View