It is subjective, what you want to modify. Better use an RNA isolation kit.
Why do you want to modify it? Do you get degraded RNA? Have you tried Trizol yet? Just make sure the tissue is homogenized well. What kind of samples are you using?
If you using vascularized tissue then make sure it will be perfused with 0.9% normal saline, then homogenized in Trizol and process according to the protocol.
Blood present in tissue have RNAase that degrades the RNA and RNA quantity obiviously decreased.
Dear Abhijeet Singh
I have a project one RNA virus and when a purification kit was used it fails to give a specific results, at the opposite site the Qiagen TRIZOL was used successfully, the problem is when i using this commercial kits or reagents i will incapable to well to studying and troubleshooting biochemical interaction, and i need to know the gradients of each chemical compound and i am using this commercial kits just as a guide.
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