Level 1 pre-post multilevel regression: Panel data or ANOVA approach?

Dear colleagues, I am currently teaching myself in multilevel analysis using educational data. I found few to zero literature on basic analysis methods. In my context we frequently use level-1...

17 February 2021 9,378 5 View

Reliable and Valid Instrument for Causes of Medical Errors?

Is there any questionnaire/survey that measures the causes of medical errors? Example: physician negligence vs. systemic issues. Looking for a reliable and validated instrument. Thank you!

15 February 2021 2,874 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Software for flow cytometry-like gating of data sets?

I am currently trying to do image based cytometry from fluorescence microscopy images. From the images I extract data such as cell size and a number of fluorescence intensities. Is there any...

09 February 2021 6,278 1 View

Can A. thaliana splice algae genes?

24 January 2021 329 2 View

Does long term preservation (10years) of Collembola in 99% Ethanol affect recovery rate of extraction by flotation?

Hello! I am currently developing an automated counting approach using Fiji to count Collembola from pitfall trapped samples that have been stored in Ethanol (99%) since 2010. I use flotation (tap...

13 January 2021 659 2 View

Co-stain Lectin PNA FITC conjugate with an uncojugated primary antibody?

Hello, I am planning to stain retinal tissue sections for IHC and want to co-stain cones and rods. For this we plan on using a Lectin PNA From Arachis hypogaea (peanut), FITC Conjugate for cones...

12 January 2021 583 1 View

How to use Abaqus USDFLD subroutine to vary stiffness?

Hi I have a problem where I am trying to vary the stiffness spatially across a structured interface. The whole idea is to vary the stiffness vertically, with a bulk stiffness of 8GPa decreasing...

06 January 2021 262 3 View

I really need help with cov-2 pseudotyped lentivirus production. Support?

Hi, i'm desesperate. i'm trying to produce lentivirus usinf BEI resources kit NR-52948. 24 hours after transfection i detect a massive GFP expression in my 293T cells. After 72 hours i filtered...

30 December 2020 9,889 6 View

How can i estimate the total phenolic content from a plant extract?

Suggestions and recommendations are welcome. A protocol used and experience would also be helpful.

25 December 2020 9,807 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

How to perform Kaplan-meier survival analysis in SPSS?

Hi everyone, This is my first time of attempting to run survival analysis. I have a data on patients with End-stage Kidney Diseases (ESKD). I would want to run survival probability by treatment...

16 February 2021 2,647 3 View

How to perform Cox proportional hazard analysis or Kaplan-meier survival analysis in SPSS?

At first please see the attachment. This experiment was performed for a period of 7 days (n=100). After 7 days, we found different level of survival rate (%) for each treatment. Now, I want to...

15 February 2021 9,145 2 View

Any model suggestion for the Electronic Health Records dataset ?

I have sequentially measured the time-series Electronic Health Record patient dataset. This is 7 years of research and each patient's blood pressure, Hba1c, LDL, Hb, Baseline Age,...were measured...

09 February 2021 4,933 8 View

How to conduct Meta-analysis of Survival Curves without Control Group?

I am conducting a Meta-analysis where I have data of only survival plots. I am stuck on performing meta-analysis on it because there is no control group for the studies. How should I perform...

07 February 2021 7,394 3 View

How can I measure speed of integration of an acquisition with secondary data?

I am currently faced with the challenge to measure integration speed of an acquisition or a merger with secondary data. Usually companies do not communicate once a target has been integrated...

27 January 2021 9,741 11 View

How can I plot survival curves for mixed effects cox models (coxme function)?

I'm working with mixed effects cox models and I can't find the way to produce a "predicted" survival curve. All I've read is about coxph objects but nothing about how to interpret the random...

04 January 2021 2,274 2 View

Don't we think use of non-functional drugs in different Nations led to genome re-engineering by Sarscov-2 virus to survive,& turns to variants ?

Re-engineering of genome my Sarscov-2 and emergence of variants now in UK, USA, South Africa, Nigeria and now upcoming in other countries.. What are the key facts and reasons ? Thank you...

31 December 2020 745 4 View

Can any one help me to solve the following error in Nimble?

19 December 2020 7,195 2 View

Are there any method to detect implanted stem cells?

Dear, We developed a microcarrier that was pre-seeded hUC-MSCs to repair a rat bone defect. However, we didn't use GFP or Luciferin to track cell survival. If there's any way to identify the...

15 December 2020 6,065 3 View