Hello everyone,
I am willing to buy a vector (luciferase) to study the LXR pathway activation in human cells. Does someone already did it and can recommend a specific luciferase vector for this kind of pathways?
Thanks in advance :)
Dear Docker community, I'm a beginner at using molecular docking, and I'm facing a problem when I want to open or drop a pdb file on Autodock tools. The folder is saved with the extension.pdb;...
05 July 2024 8,348 4 View
As a student in Bachelor degree program in computer science field we have a project in course of "Language Theory", our project related with Natural Language Processing: - First phase talks...
25 April 2024 6,961 3 View
a l instar des changements climatiques ,quelles sont les principales maladies émergentes er re émergentes au Nord africain ?
28 December 2023 4,301 1 View
2 de mes articles n apparaissent pas dans ma liste pourquoi
26 December 2023 3,700 2 View
I have the light transmission of ZnO thin films as a function of wavelength; how can I extract the refractive index from it? Thank you
11 November 2023 3,509 0 View
antiasthmatic activity of plants
02 August 2023 2,886 0 View
I want to build a song recommendation engine based on the difficulty of singing a song. I am unable to find any prior research or method of evaluation for a song. Help much appreciated.
28 November 2022 1,892 13 View
Hello everyone, I took some IF pictures for cells with zymosan A particles. The fluorescent dots seems to be inside the cells. Now I need to do a quantification for the dots to compare the amount...
07 September 2022 849 0 View
Dear experts, I'm trying to validate my model with a paper in Abaqus. The model is ground-borne vibration from passing a subway. Material hysteretic damping is the given data for damping, and I...
22 May 2021 3,181 10 View
I am using Abaqus for a project where I am modeling air propagation through buildings. I am using a 3D Eulerian domain (Eulerian elements) to represent air. I have also been trying to use adaptive...
19 May 2021 4,951 3 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...
25 June 2024 1,197 0 View
I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...
22 June 2024 6,165 2 View
In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...
19 June 2024 7,822 3 View
(Transfection with two plasmids has worked for this cell line. pRL-TK(Renilla) is not getting transfected).
16 June 2024 6,687 2 View