I tried hairy root culture in a medicinal plant. The plant roots are infected with agrobacterium rhizogenes ATCC 15834 and sub cultured in basal MS medium, after 10 to 15 days the black colour micro organism grow in infected plant parts, so I would like to want to know about that micro organism and how do control this in plant root culture. Please anybody help me.
in general fungus variety are very common in plant tissue cultures so go for the detection of fungal mass also
I think this is a Chlorella-like microalga. Try to make a wet mount and observe under the light microscope. If it is what I think, it will be quite difficult to remove from the culture; The same had happened to me when dealing with micropropagation. One of the solutions may pass through patience - as soon you have your tissues growing, take them out from the initial plate and plant them in new fresh media. Or try again :-)
Its a fungi. Add some antifungal agent in media to get rid of this.
Also try to see wether it is appearing in explant not infected with Agro. rhizo., if it is so, it is due to contamination.
it is very difficult to identify the organism just by watching the petriplate. you can go for fungal staining to confirm it as a normal contaminant. if it is so, then you can try some antifungal agent. as you mentioned its incubation and germination period is 10-15 days there is more possibility for a fungus.
thats is contamination fungi, you can try some antifungal agent or combaing antifungal...
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