Which technique is the best for mitochondrial DNA transformation?

06 June 2013 1 527 Report

Can the mutations be directed?

A method for transferring exogenous DNA molecules into isolated mitochondria by using bacterial conjugation. This includes the following steps viz (i) inserting an origin of DNA transfer (oriT) sequence into a DNA construct, (ii) transforming the construct into an appropriate Escherichia coli strain and then (iii) introducing the mobilizable DNA into mitochondria through conjugation.

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