Which subcellular protein fractionation kit would be used for insect cell?

02 February 2017 1 3K Report

Which membrane protein fractionation kit used in Drosophila S2 cell would you recommend ?

Besides, does anyone know that which fraction "nuclear membrane proteins" exist in ? membrane fraction or nuclear fraction? or both of them?

Adi Kliot

I think that for nuclear membrane proteins you should use an extraction method suitable for membrane proteins (as it we give you proteins from both the outer membrane and the nuclear membrane).

I'm afraid I've never used a kit for this type of extraction..

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