DOes someone know what is the best basis set and method for calculating excited state of ion CH5+ ??

I've been trying to do my calculations related to optimization of excited state of ion CH5+ using method TD-DFT with M06-2X and aug-cc--pVTZ. My input file is as shown...

03 January 2021 2,148 0 View

Can I ignore the NMAR assumption when missing data is very negligible?

Hello! I am performing preliminary analyses on my data set (N= 187 heterosexual couples). When examining the summary of missing values, SPSS indicates that only 0.16% of my data is missing....

01 December 2020 5,562 13 View

Likert scale over repeated measures?

I am working on research where I have 66 participants who perform an exercise every day, and then they need to respond to 14 questions with a Likert scale (1-10). They performed this exercise for...

20 November 2020 4,682 6 View

Which anesthesia system should I buy to perform stereotaxic surgeries in mice?

I am trying to start a protocol involving bilateral cannula implantation in specific brain regions in mice (mPFC, hippocampus). For that, I need to buy an anesthesia system. I found many...

06 November 2020 3,613 3 View

Emission filter for FLIM to filter off GFP signal?

Dear all, I am measuring changes in fluorescence lifetime of my chromophore in cells expressing also GFP and I would need to filter off the GFP signal. After playing with the parameters in...

11 August 2019 5,297 3 View

Testing for correlation/regression between two scale scores on SPSS?

I am trying to find the correlation/regression between these two variables: Social media usage (measured by 10 items) (total scale score 50) Materialism (measured by 9 items) (total scale score...

10 April 2019 4,123 4 View

Can you recommend any peptide-coated microplates?

Hi! Can you recommend any company (or at least a research-team) where I can order a microplate covered with peptides according to my needs? I need it for immunological assays. Thanks for all...

12 February 2019 5,959 2 View

Can dance/movement be a language? What are the effects of different languages on movement quality and understanding of movement?

I am researching whether dance can be considered a language, and different languages effects on movement quality. i am interested in how dancing to spoken word, interpreting it affects dancers...

14 August 2018 4,051 11 View

How to isolate DNA from water?

What is the best way to isolate genomic DNA from water? Using the ready kit centrifuge a sample of water to obtain the sediment of microorganisms or maybe filter water and isolate DNA from the...

07 August 2018 2,397 8 View

Does anyone have MAD109 (M109) cell line and would be able to share?

I am facing a problem I can not find where to purchase MAD109 (M109) cells. Would someone be so kind and help me or even share it with me?

28 March 2018 9,101 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View