Dear Researchers
I have done in vitro hepatoprotective activity of diff. conc. of my plant extract in HePG2 cell lines using Paracetamol as toxicity inducer and silymarin as reference drug. I am in confusion to use which method to use for statistical analysis of data. Whether two way ANNOVA, one way ANNOVA need to be used or independent t-test is sufficient for analysis? Please guide me regarding this.
If you are comparing your groups as treated versus control you can use t test
I assumed, your experiment based on the different concentrations of your plant extract how effect on suppressing the toxicity induced by Paracetamol. Then you have two different variables,
Ex your plant extract concentration and Inducer's concentrations.
You should perform one-way ANOVA in this experiment if you do not change the inducer's concentration. If you change both concentrations you should check with two-way nova, because both are then independent variable.
A Two Way ANOVA is an extension of the One Way ANOVA. With a One Way, you have one independent variable affecting a dependent variable. With a Two Way ANOVA, there are two independents. Use a two way ANOVA when you have one measurement variable (i.e. a quantitative variable) and two nominal variables.
- A one way ANOVA will tell you that at least two groups were different from each other.
-The results from a Two Way ANOVA will calculate a main effect and an interaction effect.
https://www.qimacros.com/hypothesis-testing/two-way-anova-with-replication/
1. Independent t-test: compare untreated control to either one of your treatment group.
For instance: Paracetamol vs. Plant extract concentration 1 or, Silymarin vs. Plant extract concentration 1.
2. One way ANOVA with Dunnett's post-hoc test: compare untreated control to all the treatment group.
For instance: Untreated control (Normal control) vs. Plant extract concentration 1, 2, 3 and so forth.
OR,
One way ANOVA with Tukey's post-hoc test: compare all the treatment groups in your in vitro study.
3. Two way ANOVA - inappropriate in your scenario as what you mentioned doesn't need this analysis.
Hope it's helpful. All best luck!
If you have more than two group go for ANOVA and other Post hoc test. my new video on ANOVA in efficacy Studies using SPSS
https://youtu.be/76L9zS0uJoA