Many groups are nowadays trying to stimulate nervous tissue electrically or optically to achieve some intended therapeutical results. 

However, the usual staining methods for microglia or astrocyte activation  seem at odds with a short term overstimulation (kind of single exposure...). In acute settings, the body doesn't have the time to activate these pathes....

Does anybody have a recommendation how to quantify nerve or tissue damage below the threshold of literally "cooking" or "carbonizing" tissue? (That would be easy to recognize, though...) 

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