Many groups are nowadays trying to stimulate nervous tissue electrically or optically to achieve some intended therapeutical results.
However, the usual staining methods for microglia or astrocyte activation seem at odds with a short term overstimulation (kind of single exposure...). In acute settings, the body doesn't have the time to activate these pathes....
Does anybody have a recommendation how to quantify nerve or tissue damage below the threshold of literally "cooking" or "carbonizing" tissue? (That would be easy to recognize, though...)
Look into graded potentials, if you can study its conduction in the same time.
Dear Ulrich, I suggest the Cleaved Caspase-3 staining of apoptosis pathway. Here I recomend to you an paper where was evaluated the neuronal apoptosis in acute fase of lesion (6h after lesion), double labeling neurons with Cleaved Caspase-3.
http://www.sciencedirect.com/science/article/pii/S0006899317301233
Good Luck!
Thanks for the suggestions! In fact, we perform potential recordings anyways - but I wanted to have an independent measure of short term damage upon stimulation. My impression is, that even though there are still some evoked potentials to be measured, there is damage to be expected. What about looking for early gene expression? Any suggestion for that?
Wells, J. D., S. Thomsen, P. Whitaker, E. D. Jansen, C. C. Kao, P. E. Konrad and A. Mahadevan-Jansen (2007). "Optically mediated nerve stimulation: Identification of injury thresholds." Lasers Surg Med 39(6): 513-526.
uses "hematoxylin and eosin (H&E) to demonstrate pathologic changes in the tissue. Selected unstained slides were submitted for Luxol fast blue-Periodic acid Schiff stains (LFB-PAS) to highlight myelin and collagenous connective tissues. " "thermal injury, denoted by birefringence loss, can be detected in H&E-stained sections, the resolution of subtle changes is significantly improved when sections are stained with dyes that enhance the tissues’ natural birefringence. For example, picrosirius red (PSR) enhances collagen’s birefringence and has been shown to enable detection of thermal injury in collagen and muscle. "
- Badges
- Science topic