Which soil DNA extraction kits do you recommend for evaluation of arbuscular mycorrhizal fungus diversity?

More Paulo Emílio Lovato's questions See All

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Baseline drift in HPLC? What causes this?

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I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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Does crude extraction using NaOH and Tris work well with Fungi?

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Sachinandan De

After removing the sand and soil particles to a maximum possible extent you can use any standard DNA isolation kit.

Javier Parladé

Hi Paulo. The Norgen Soil Kit works well, with a DNA yield similar to MoBio (I checked a free Norgen commercial sample and compared both kits). However, I find the Norgen protocol a little bit more long. If the price is ok, it is a good option.

Best regards!