Which soil DNA extraction kits do you recommend for evaluation of arbuscular mycorrhizal fungus diversity?

More Paulo Emílio Lovato's questions See All

Any suggestion of free of charge questionnaires that measure mood states?

I need a free of charge and short questionnaire that measures mood states. The questionnaire will be used as in a neuropsychological evaluation study. It is well known that mood states can...

16 January 2021 8,284 4 View

Why IPNI voluntarily abbreviates some people's surnames in their standard forms?

The International Plant Name Index is known for standardising plant scientific names, including the standard forms of authors' names. I heard that one person's standard form may be chosen when...

01 January 2021 1,928 5 View

What are the largest scientific paper repository (e.g. Web of Science, Scopus) in the world? Is there a website that shows this classification?

I would like to know a hierarchy of the five largest scientific journal articles bases, for example.

21 September 2020 1,768 9 View

Have you considered publishing your research on COVID-19 in SEMINA: Exact and Technological Sciences??

I am an editor at Semina: Exact and Technological Sciences, an open, no fees journal, maintained by the State University of Londrina, Brazil. See journal information at:...

06 September 2020 3,510 14 View

Glass or PVC material to perform a soil leaching experiment of pharmaceuticals?

Hello I am developing a soil column experiment to study pharmaceuticals transport and attenuation.I would like to know If is better to use a glass or a PVC column to perform this experiment and...

10 August 2020 6,214 3 View

How do I reduce basal phosphorylation in PBMCs for phosphoFlow detection?

I'm measuring the phosphorylation status of different proteins (e.g. NFKB, ERK, AKT1, p38 etc) using phosphoflow cytometry. The protocol for surface and intracellular staining works well, all the...

22 July 2020 3,233 8 View

Hi! Someone has installed Pardiso library to work with Matlab on Linux?

I am having some problems when I try run a program with Pardiso. I seems that the Pardiso has problems with the BLAS library from Matlab. Is it correct?

28 May 2020 1,276 5 View

How to make the student participate in the virtual classroom during the COVID-19 pandemic?

We are facing a difficult problem during the pandemic: the number of students attending virtual classes has decreased significantly at the undergraduate level.

20 May 2020 5,122 27 View

How sure are you, that after TUR-B for NMIBC, to have enough muscle tissue for correct histopathological examination?

Deep resection for correct histopathological analysis in TUR-B can be challenging for the surgeon with a low to mid-experience level. This leads to inadequate analysis, possibly postponing radical...

11 April 2020 5,713 3 View

How are you guys dealing with quarantine in terms of fieldwork and laboratory analysis?

In my case, there are some studies that my team and I need to collect data monthly in situ for laboratory analysis (major ions and stable isotopes), such as rainwater, surface water, and...

24 March 2020 7,046 10 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Sachinandan De

After removing the sand and soil particles to a maximum possible extent you can use any standard DNA isolation kit.

Javier Parladé

Hi Paulo. The Norgen Soil Kit works well, with a DNA yield similar to MoBio (I checked a free Norgen commercial sample and compared both kits). However, I find the Norgen protocol a little bit more long. If the price is ok, it is a good option.

Best regards!