How to calculate the electromagnetic field strength within 2km depth from the surface?

Not by simplified equation. If modeling is required, which program would you recommend?

19 September 2018 1,469 2 View

Has anyone tried to get rough estimation of concentration of a PCR band with Promega 1kb ladder?

I know it is not made for quantification but do you have any empirical data for that? Specifically, I would like to know: if I load 6 ul of the ladder, the 1000bp band of the ladder will...

12 June 2018 4,165 10 View

Does absorptivity and emissivity depend on temperature?

Hi, I am wondering, if absorptivity and emissivity coefficients depend on the temperature of a body? Example I have two plates, plate 1 (T1=300 Kelvin) and plate 2 (T2=301K) Both plates are not...

21 March 2018 6,224 1 View

I would like to extract second order statistical texture features from the approximation coefficients of the 2D-DWT.

Is it possible to calculate energy, contrast, homogeneity, etc by using approximate coefficients of 2D-DWT and the histogram of LL Band image of 2D-DWT. I didn't use GLCM for some reasons.

02 March 2017 2,987 2 View

When isolating CD133+ population from GBM cell line, which isoform do I need to use for CD133?

17 June 2016 4,371 2 View

How can I establish a wnt-3a conditioned medium from Hek293 cells?

In my job, I transient transfected Active-wnt3a construct into Hek293T and Hek293 for expression , I collected three batch(fresh medium 5ml in 10cm dish for 24hr) conditioned medium...

23 February 2016 9,386 5 View

Can anyone assist with finding a protocol for manual dissociation of RPMI 8226 tumors for single cell suspensions?

25 August 2014 5,773 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View