Liebermann Burchard reagent is used for steroid in TLC,but now acetic anhydride is band, so which reagent will use for steroid isolation in TLC ?
Liebermann Burhard reagent is used steroids having hydroxy groups or any other such group where acetylation is possible.
In general, we use Ceric sulphate reagent for steroids, terpenes, and terpenoids.
See Reagent # 57 in the attached file.
vanilline - sulfuric acid 1g vanilline + 4 ml k. H2SO4 to 100 ml with ethanol
Thank you Ivan Sir and Ghazanfar Sir.
Thank you Saikat Sir and Aldallal Sir.
vanillinsulphuric acid. pink color or 50 % ethanolic sulphuric acid, but specific test is lieberman only
1 : 1 mixture of a solution of tetrazolium blue (0.25 mg) in methanol (50 mL) and a solution of sodium hydroxide (10 mg) in water (50 mL)
Thank you Chauhan sir, for the reply.
kindly reply me. Thanking you in advance.
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or we can collaborate
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