Trouble shooting with his tagged protein expression in E.Coli?

i have issues in expressing a chicken protein in BL21 cells with his tag. I do not see induction even at 18 degrees. Any suggestions

15 February 2021 8,939 3 View

Any thumb rule to know the surface charge of bacterial cellwall?

I would like to prepare a list of the bacteria with the positive charge on cellwall. Can anybody suggest me about the resource for such details. Is there any thumb rule to determine the charge of...

04 November 2014 7,391 1 View

protein purification-IPTG stability

can any tell me how stable is IPTG stock. i have made a stock of 0.5M IPTG one year back, can i use it now regards aparna

02 March 2010 6,478 1 View

Do nano dispersion PL emission wavelength keeps changing by varying the concentration of the nano dispersion?

According to my knowledge, I knew that by varying the sample concentration one can vary the intensity of the PL emission, but in my sample, the PL emission wavelength keeps varying by changing the...

01 January 1970 7,738 4 View

I still wonder why MgB2 based nano dispersion that appears golden yellow in color absorbs only in the UV region (< 300nm) ?

01 January 1970 2,496 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

How does an AC-DC power supply Flyback Converter Based USB Charger Model using Matlab/Simulink looks like?

I am required to learn about Flyback converters and I got stuck not knowing to full design of the power supply flyback converter Based USB Charger Model using Simulink, especially the design model...

25 February 2021 5,435 2 View

How do you mesh a thin solid in Fluent?

Hello, I have used the shell function on SpaceClaim to create thin solids for the model I am studying. These are contained within an enclosure for my study. I chose this approach as it avoids the...

24 February 2021 8,856 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software?

Hi every one Is it possible to define biogas as a fuel in the reformer process(scwg) in Homer software? Biogas fuel is not available in the options thanks

23 February 2021 6,518 3 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

How to build fouling and erosion simulation model for Aero Engine compressor and turbine rotor in Simulink / Matlab?

How to build fouling and erosion simulation model for Aero Engine compressor and turbine rotor in Simulink / Matlab?

22 February 2021 3,697 1 View