First level of this assay needs Heparin pre-treatment to release tissue bound LPL and then either immunoassay is possible, however recently development has taken place to check LPL activity based on fluorescent labeled substrate (TG) along with quencher and on active cleavage by the enzyme the quencher and the labeled product dissociates to give signal of activity.

This test might prove useful for hypertriglycerdimia work-up. Since Insulin is a positive regulator of this enzyme so in diabetic patients a low activity but not low quantity (as reported by immunoassay) will be observed. Occasionally ApoCII which also an modulator should be checked along with.

Thank you for suggestion.