Hi all,
I am a beginner in cryoEM field.
I know sometimes we must remove his, flag, strep, MBP, eGFP in X-ray crystallography study.
However, when I read papers, I found that these tags were not necessary to be removed in cryo-EM study. So I am wondering if we can keep all the tags for cryoEM study, especially the big tags such as MBP and eGFP tag, but will they affect the protein real conformation I mean in vivo conformation? I didn't remember who told me that MBP connects to proteins through a flexible linker, so I am not sure if we can see the big tag density in this case.
On the other hand, these big tags can help to increase the protein particle size, and may also help to improve the orientation preference in cryoEM study.
Are there any cases or publications that solved cryoEM structure containing big tags e.g. eGFP or MBP tag?
Any comments or suggestions are welcome, thanks!
here is one, there may be many more here: https://www.ebi.ac.uk/emdb/search/maltose%20binding%20protein
- Badges
- Science topic
- Similar topics
- Geoscience
- Asia
- India