Zixun Han

6 Questions 5 Answers 0 Followers

Questions related from Zixun Han

How to use the detergent CHS?

When people purify membrane protein, why they used LMNG together with CHS, or DDM together with CHS? But there is no CHS when using GDN?

16 March 2023 5,496 0 View

Which protein tags does not need to be cleavaged for cryo EM structure determination?

Hi all, I am a beginner in cryoEM field. I know sometimes we must remove his, flag, strep, MBP, eGFP in X-ray crystallography study. However, when I read papers, I found that these tags were...

24 February 2023 5,325 1 View

How to thaw protein after taking out from -80 degrees?

I have seen some conflicting advice on this point. I always add 10% or 5% glycerol before flash freezing with liquid nitrogen. when i take out the protein from -80c freezer, i put it on ice or ice...

14 July 2016 3,505 3 View

Does "different origins of replication" is essential in co-expression?

recently i learn some knowledge about co-expression. the EMBL website said, in the case of co-expression from different vectors, To ensure plasmid stability, the vectors should have:(1)different...

29 June 2016 1,783 3 View

Can high speed centrifugation affect protein-protein interaction?

Based on GST pull down assay, my protein A and protein B has interaction. i mix A and B and put in 4℃ overnight, centrifuge at 20,000g for 20min, then load on gel filtration chromatography with...

25 September 2015 7,856 6 View

How could I check whether there is RNA in my protein solution?

I purified a recombinant RNA binding protein from E.coli, I worried that my RNA-binding protein was contaminated with host RNA during purification. I need to remove RNA completely, I have treated...

07 September 2015 9,543 12 View