We are interested to investigate the effect of different anti CD38 on the level of ectoenzymatic activity in different myeloma cell lines. We have the Fluorimetric cADP-Ribose Assay Kit (999999-99-4) sigma Aldrich. We lysis the cells using RIPA buffer.
We would like to know if there another method rather than using RIPA buffer.
Nick Chacos
Since RIPA contains SDS, using it is going to denature a lot of the enzyme activity present. I would try something much gentler like Triton, NP-40, etc. It doesn't take a lot to lyse mammalian cells and those would preserve enzyme activity.
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