hello i have question regarding intranasal drug delivery as drug delivered to the brain through nasal route will either bypass BBB (olfactory region) and/or remaining drug will either cleared by mucocilliary clearance or will go into the systemic circulation so i have que that during in-vitro permeability study (franz diffusion cell) which media should i use in acceptar campartment in order to mimic its permeation in brain & blood as well.......
Hi,
These references should be useful, you will find cell culture models or excised nasal tissue as well as buffer systems used for permeation studies. It seems that oxygenated phosphate buffered glucose enriched solutions are of interest.
M.Christiane Schmidt, Hagen Peter,Steffen R Lang,Günter Ditzinger,Hans P Merkle. In vitro cell models to study nasal mucosal permeability and metabolism. Advanced Drug Delivery Reviews, volume 29, Issues 1–2, 5 January 1998, Pages 51–79
P.M. Reardon, In vitro nasal models, in: R.T. Borchardt, P.L. Smith, G. Wilson (Eds.), Models for Assessing Drug Absorption and Metabolism, Plenum Press, New York, 1996, pp. 309–323
Best regards,
I have used the Ph7.4 buffer, from your question i understand you are trying to mix buffers to represent both the neurological pathways to brain and noramla pathway to blood,
unfortunately i dont beleive there is any way you can mimic this by franz diffusion cell,
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