For RNA extraction from Wistar rat adipose tissue to study genes involved in fat oxidation, it is best to use the solid or powdery frozen part of the sample after grinding with liquid nitrogen, and avoid the oily layer. RNA is found in the cells, not in the oil, and excess oil can interfere with extraction and reduce RNA quality. Work quickly with pre-chilled tools to minimize fat melting and contamination.

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Raghad Mouhamad