Which one is more reliable between Cleaved Caspase-3 assay (Western blot) and Caspase Glo 3/7 assay (Luminescence)?

Zulfiker Rahman Khondoker @Zulfiker_Rahman_Khondoker

03 March 2018 1 10K Report

I would like to determine the apoptosis in HeLa cells after treatment with some drugs. Scientist are being used Cleaved Caspase-3 as well as Caspase Glo 3/7 assay for determining apoptosis.

Does anybody have idea/experience regarding which of the above techniques is more reliable to determine apoptosis.

Md. Mostafizur Rahman

I usually use WB to detect the Cleaved caspase 3. I believe, the WB is an well accepted and reliable method for protein level assay. In most of the recent scientific articles WB is used to determine protein expression/activity assays. Thank you. Best wishes!!!!

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