Sunha Hwang

2 Questions 2 Answers 0 Followers

Questions related from Sunha Hwang

Which one is best protocl for Thp-1 differentiation?

I found two papers to establish a Thp-1 differentiation protocol (Thp-1 -(PMA)-> M0 -(LPS+IFN or IL-4+IL-13)->M1 or M2). I want to compare both protocols, but the PMA priming times and the types...

13 September 2023 1,152 0 View

Why are proteins precipitated after elution (ni-nta affinity elution)?

Hi, I try to purify protein expressed HEK293T I've tried many things, but I failed... * Binding: 0 mM Imidazole, Wash: 20 mM Imidazole, Elution: 500 mM imidazole * All buffer contain protease...

14 February 2023 3,806 5 View