Oil Red

Oil red

Thanks Christoph and Apostolos

Others have already answered ... and I agree with them: Oil Red

I agree oil red is good

Oil red

Oil redO is the most popular techniques for staining fats.But during long storage of stained sections(in my expierence itwas about 0.5-1 year),they loose their colour.I prefer Sudan Black.

I always use Sudan black

But still oil red is the better choice

Please find attached what considered to be the best prepared kits for fat and lipid stains with references

Dear Ahmed,

everybody has his/her best "pet"!

And: results are the discriminating value for choosing this one or that one...

But: for me not only in the case of citing and posting commercial sources I think it would be honest and GSP () to cite also the source from which you are citing (or copying) (even if from the hidden URL's in the text one can see from where it was taken).

In the case of your posted file this should be (not only for copyright reason! cf.: > : http://www.emsdiasum.com or, more precisely http://www.emsdiasum.com/microscopy/products/histology/lipid_fat_stains.aspx

Also, IMHO, a personal disclaimer (e.g. , etc.) should be added.

Best regards, Wolfgang

I totally agree and thank you very much for this comment and I must say I have no financial interest or affillation or any other interests

Wolfgang and Ahmed, Thanks for your contributions to my question

Hi F.M. Onyije

Thank you very much and you are always welcomed

I have no experience with Sudan Black but it is worthwhile to try it

Dear Ahmed, dear F.M. Onyije,

your recent posts for me are welcome too. I would like to add here only that there have been o lot of other posting threads on the issue "Lipid&Fat Staining" (guessing you want to stain sections from either Formaldehyde fixed tissue (or cryosections too, the latter would imply to think over again about "the best" staining dye), but -as stated very often in these other threads - and quality of fat/lipid stainings always depend on the "real" retention of those substances in fixed tissue material (since most of it will be lost during processing).

cf, e.g.(not exhausting): https://www.researchgate.net/post/Is_there_any_suitable_staining_method_protocol_for_lipids_in_paraffin_sections or

http://www.researchgate.net/post/Does_anyone_know_of_a_good_histological_staining_for_brown_adipose_tissue_in_fresh_frozen_sections

Best wishes and regards,

Wolfgang

Hi Wolfgang Muss

Thank you very much for this comment and rightly raising the point fixed and fresh specimens . Best wishes

You're welcome, dear Ahmed,

best regards,

Wolfgang

, dear Wolfgang

Thanks with my best wishes