I am trying with the 60% (w/v) (acrylamide: bisacrylamide) 19:1 ratio for the preparation of resolving gel and the 49:1 ratio for the preparation of stacking gel. It is not working well. For the gel staining, I am using the azure A dye (0.08%) and the time I used 1 hour. After staining, I used deionized water for the destaining of the gel, but I noticed the gel became dark, and the band couldn't notice.

What could be the solution to solve this problem? I reduced the staining time to 20 minutes and diluted the staining percentage (0.08% to 0.03%), but I still have the same problem. Does anyone who has experience can assist me regarding this issue?