Which % of acrylamide and bisacrylamide ratio will be suitable for the crude and standard glycosaminoglycans (e.g. CS, Heparin) native PAGE analysis?

I am trying with the 60% (w/v) (acrylamide: bisacrylamide) 19:1 ratio for the preparation of resolving gel and the 49:1 ratio for the preparation of stacking gel. It is not working well. For the...

13 August 2024 2,555 0 View