The choice between hydrodistillation and steam distillation depends on various factors, such as the plant material being used, the desired quality of the oil, the constituents being targeted for extraction, and the sensitivity of these constituents to heat.

Hydrodistillation is preferred in some cases because:

However, steam distillation is preferred for:

Thank you for this clarification Sara Gonçalves

At present and in the recent decade, one of the best available methods for extracting natural bioactive compounds and secondary metabolites from genuine/original germplasms is the enzyme-assisted-extraction method. Of course, This method has a very high costs.

https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/enzyme-assisted-extraction#:~:text=EAE%20works%20on%20the%20principle,to%20release%20the%20intracellular%20components.

https://www.researchgate.net/publication/258212363_Enzyme_-Assisted_extraction_of_Bioingredients

Regards

Yes Hamid Reza Farhang, but its cost may be a barrier for laboratories to perform this method...

Stem distillation and extraction with solvents may be appropriate solutions.

However, it depends on the material you are dealing with.

In ant case, purification by fractional distillation and/or column chromatography may be necessary.