Serum volume will be 10 to 100 microliter
Dear Rakesh,
there are some calcium-sensitive fluorescent dyes around. https://en.wikipedia.org/wiki/Calcium_imaging
Concentrations of Ca in serum could be determined colorimetrically by
the o-cresolphthalein complexone method.
1. Calcium Detection Kit (Colorimetric) (abcam)
2. Calcium Colorimetric Assay Kit (Biovision)
Thank you all for valuable suggestion
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