We have very low amount of human mRNA (from 5 to 120 ng). Which method could we use to quantify mRNA ?
Seems like you already know the quanity. Can you be more specific?
Hi folks, I am a protein crystallographer. A colleague of mine (chemist) obtained some processed diffraction data together with a "SHELXS" solution. So she turned to me for help to follow this up....
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Teat End Hyperkeratosis generally occurs in machine milked cows as a response of teat end tissue resistance to high vacuum of milking machines and it reaches the peak score during the fourth month...
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Any province in the country but the research focus is western cape but would like to know what others are experiencing from other countries.
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Folks: I have tried to contact the BIOVIA DiscoveryStudio support team, somehow my browser does not allow me to open their "contact form". I am trying to visualize and perform an analysis on...
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I am looking for a supplier who sells lipopolysaccharides (LPS) from Acinetobacter baumannii. I have not found one... Thank you for your help
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Hello, What do you usually use when you have to fix optical cristals on a holder before send it for UHV metal deposition?
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I am thinking of pursuing a PhD but confused in choosing a good topic. What are today's most attractive and relevant topics in Library and Information science most especially in the field of ICT,...
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What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
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How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
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Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...
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What happens in snapTotal- seq? (Single-cell Total-RNA Profiling Unveils Regulatory Hubs of Transcription Factors https://www.nature.com/articles/s41467-024-50291-3) In this protocol, several RT...
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I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...
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Hello, I'm generating stable cell lines by transfecting plasmid DNA with lipofectamine 3000. The cells went through antibiotics selection for 3 weeks (polyclonal selection) and when I analyzed...
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I plan to perform a t-test between risk index and leakage values to know if there is a significant difference between leakage values of low, medium, and high risk pipes. However, the data I have...
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Hi, I'm a graduate student, college of nursing, chungnam national university in Daejeon. The topic of my thesis is "Quality Assessment of Tools for Evaluating Acute Postoperative Pain." I have...
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For additional information, I am a master's student looking for a LCA software to do my thesis. So a less expensive one is my first priority, but if it is not able to help me with a clarity...
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Is it very literally subbing in shannon wiener index values instead of species abundances?
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