Blood glucose can be easily determined as a quantity using various methods but I can find any method authentic to separate them from blood. for qualitative approach I need to separate them.
Dear Rashadul,
The following paper covers the answer to your question.
Biochemical Medicine
Volume 6, Issue 2, April 1972, Pages 160-165
An improved method for the isolation of labeled blood glucose as potassium gluconate and the determination of its radioactive specific activity
Author links open the overlay panel. Numbers correspond to the affiliation list which can be exposed by using the show more link.Reuben Malimovka ∗
doi:10.1016/0006-2944(72)90033-6
Abstract
A practical procedure for obtaining an alcoholic protein-free filtrate from blood (or plasma) and determining its glucose content is described.
Isolation of glucose from this filtrate was carried out by the preparation and crystallization of potassium gluconate. The radioactive specific activity of the isolated salt was determined by liquid scintillation counting in an acid scintillator solution, in which samples containing up to 50 mg potassium gluconate were dissolved.
The technical procedures described in this paper have been used in this laboratory, in routine work, for more than 3 years.
Hoping this will be helpful,
Rafik
- Badges
- Science topic
- Similar topics
- Correction