You can go for Walkley and Black's method.

Walkley black is better and simple method. H202 is poor exactly is more exploratorie data

Walkley and Black, 1934 has been widely tested and has wide applications than H2O2 with minimal error.

walkley and Black

I still use the dichromate acid oxidation procedure of Walkley black.

We still use Walkley and Black method so far with an acceptable data results

For determination of organic carbon walkley and black method is simple, by this method only 77% of the total oxidizable organic carbon is oxidized. So total organic carbon is find out by e.o.c.% * 1.3 = % total o.c.

The oxidation efficiecy is related to the soil type and components (see also Walkley, 1947). I would go for WCO using a sulfochromic acid (Houba et al., 1997). Difference between W&B is the heating step (135 C, 1 hour). See also Walinga et al., 1992. The heating step is crucial to attain complete oxidation.

For the determining SOM I'd use loss-on-ignition + use of clay correction factor, iron oxide correction factor.

I'd forget chemical oxidation approaches, to messy and expensive. Provided you don't have too much carbonate ( remove first) you should try combustion with a co2 analyser

Walkley black method is better

Ya, Walkley & black method is good, which give about 77% recovery.

Well, Walkley and Black method has been in use for long. But i just tried the ignition method by Reddy et al (2009) and it works perfectly. Just ash a known quantity of the soil sample between 450-650 degree and calculate the loss on ignition. The percentage loss on ignition gives the total OM content. Cheers

There's growing interest in the use of FTIR and other spectrometry techniques to examine SOM though this is in its preliminary stages

Boyd, 1995 is the easiest one.

I also use LOI, and got very similar results to the few correlation samples I did with the walkley black.

Momin..I tried to find Boyd (1995) and couldn't come up with anything. What is that method?

Walkley & Black's method

REFERENCES

1. Jackson, M. L. 1958. Soil Chemical Analysis. 214-221.

2. Walkley, A. 1947. A Critical Examination of a Rapid Method for Determination of Organic Carbon in Soils - Effect of Variations in Digestion Conditions and of Inorganic Soil Constituents. Soil Sci. 63:251-257.

3. Walkley, A. and I. A. Black. 1934. An Examination of Degtjareff Method for Determining Soil Organic Matter and a Proposed Modification of the Chromic Acid Titration Method. Soil Sci. 37:29-37.

4. Schollenberger, C. J. 1927. A Rapid Approximate Method for Determining Soil Organic Matter. Soil Sci. 24:65-68.

I do agree with Pere Rovira's answer (a) with a good SOC recovery.

LOI on ignition is very straightforward if you have access to a muffle furnace, but as mentioned is associated with various errors, usually over-estimation due to loss of bound water. In my experience LOI is more suited to soils rather than sediments with low organic content. The 'gold' standard appears to be combustion to CO2 in a CuO furnace (900 degree) and IR detection of evolved CO2 i.e. commercially available elemental analysers. Acid treatment prior to analysis allows TOC content to be measured but you may need a hydrogen filter installed to the EA to protons added during acid treatment. I have also tried titration methods and found them quite laborious and less accurate. However the consumables and equipment is easy to get a hold of. I would say it depends on how critical the data will be and to what level of accuracy - if it is complimentary data and accuracy to a number of dec places is not critical then I would have no issue with LOI and wet chemistry methods.

As for soil organic matter to a molecular level - solid state 13C NMR as well as analysis of molecular classes especially lipid classes and lignin phenols is the way to go. This is common yet quite specialised work however.

Hello Muhammad,

you can go through the article to get clear about your doubts that which methods you should be using for estimation of soil organic carbon.

from my point of view, there are two main methods to determine the organic matter. the first is an oxidative reaction named Walkley & Black where you must use several reactive substances, the drawback of this method is that only will obtain a 77% of the total reaction. Second method commonly used is known as lost on fire, this method determine the total organic carbon and needs a higher temperature (540 ºC), after must multiply the outcomes by a factor to obtaina organic matter content.

As we know that generally soil oganic carbon is 58% of soil organic method. so by calculating Soil organic carbon by walkley and Black method we can calculate Soil organic carbon.

procedure

reagent

1. Potassium Dichromate: K2Cr2O7

2. Ferrous Ammonium Sulfate: Fe(NH4)2(SO4)26H2O

3. Sulfuric Acid: H2SO4

4. Phosphoric Acid: H3PO4

5. Sodium Fluoride: NaF

6. Diphenylamine: C6H5NHC6H5

SOLUTIONS

1. 1N Potassium Dichromate: a. Weigh 49.04 g potassium dichromate (previously dried for 2 hours at 100 C) into a 1 liter volumetric flask. Dissolve and dilute to volume with deionized water and mix well.

2. 0.5N Ferrous Ammonium Sulfate

a. Slowly add 20 ml. sulfuric acid to a 1 liter volumetric flask containing 800 ml. deionized water.

b. Add 196.1 g ferrous ammonium sulfate. Dissolve, dilute to volume with deionized water, and mix well.

c. Prepare daily.

3. Diphenylamine Indicator:

a. Dissolve 0.500 g diphenylamine in 20 ml. deionized water.

b. Slowly add 100 ml. sulfuric acid. Carefully mix with a glass stirring rod. CAUTION: this solution is corrosive and can cause sever burns. Proper precautions are given on the MSDS sheet for sulfuric acid.

PROCEDURE

1. Weigh 1.00 g soil into a 500 ml. erlenmeyer flask.

2. Add 10 ml. of 1N potassium dichromate solution.

3. Add 20 ml. sulfuric acid and mix by gentle rotation for 1 minute, taking care to avoid throwing soil up onto the sides of the flask. Let stand for 30 minutes.

5. Dilute to 200 ml. with deionized water.

6. Add 10 ml. phosphoric acid, 0.2g ammonium fluoride, and 10 drops diphenylamine indicator.

7. Titrate with 0.5N ferrous ammonium sulfate solution until the color changes from dull green to a turbid blue. Add the titrating solution drop by drop until the end point is reached when the color shifts to a brilliant green.

8. Prepare and titrate a blank in the same manner.

9. Prepare one duplicate sample and one quality control sample with each set of samples analyzed.

CALCULATION

1. % Organic Matter = 10[1(S÷B)] X 0.67

S = sample titration

B = blank titration

Dear pere may i know whats problem associated with Walkley-Black method.

I will determine the organic carbon through the determination of the total carbon with the elementar analyzer (CHN). Than you should determine the C resulting from the CaCO3 and make the difference between them to obtain the organic carbon!

Thanks sir.....

Mr Pere Could you upload the file

Nelson, D.W., Sommers, L.E., 1996. Total carbon, organic carbon, and

organic matter. In: Sparks, D.L., (Ed.), Methods of Soil Analysis. Part 3.

Chemical Methods, ASA-SSSA, Madison, USA, pp. 961–1010.

LOI or C/N analyzer

Thanks Pere

Combustion for elemental analyses is cheaper now and it will give you something more than just carbon. combustion will also release inorganic carbon though. Acid digestion should take care of that but avoid HCl as Cl- is not a friend to the columns of elemental analyzer. If interested in N content of soils as well, i would suggest a zero blank autosampler, to prevent air N2 contamination.

It depends from your money. Dry methods are better (conductivity or IR detection of CO2). Wet methods have problem, the popular DICHROMATE methods yields a lot of contamination, better the PERMANGANATE one, but both methods give errors. Also it depends of the amounts of organic matter you have in the sample. I include my list of publications where you can select some papers related on. You can obtain these here or in Academic.edu.

I have calculated organic carbon percentage using Walkley and Black Method while organic matter content using loss on ignition method. You can also use Walkley and Black Method to estimate soil organic carbon. Since, soil organic matter contains 58% of organic carbon, the percentage of organic carbon multiplied by 100/58=1.724 which gives percentage by organic matter

i.e. organic matter= organic carbon x1.724

You can use Walkley and Black method for determination of soil organic carbon.

you can use the Walkley and Black, chromic oxidation procedure.

Hi,

You can use the Anne method. Wet oxidation  with sulfur acid and bichromat of potassium.

I prefer using dry ashing of soil using furance

I prefer using dry ashing of soil using muffle furnace

Wakley Black methods (wet digestion with potassium dichromat ) . I used it for different soils. i think is one of the best accurate methods.

in this one they made a nice comparison, like mentioned in earlier comments this article suggest to add heating to ensure complete oxidation (Mebius method), the elemental analysis is reported as most accurate but then you only have total carbon of course, but it might be additionally interesting to check for the reliability of any of the method you will choose

I think that Walkley-Black method of organic carbon analysis in soil is more accurate as compared to combustion method, where the latter method gives high value of organic matter

 Chatterjee,A and others paper attached by Dr.( Ms.)Sudeshna provides very good comparison of various available methods.

In my opinion, Walkley and Black method will be more reliable.

I agree with the answer given by Kamal Hama Karim.

Walkley and Black's method is good as it is simple and less timing one. it is widly used method.

you can use CHNS analyzer for most accurately C determination.

H2O2 oxidation method was one of the earlist methods used to estimate soil organic carbon.But has not become popular  as Walkey and Black wet oxidation method because of some limitations. The two  problems of wet oxidation method  are incomplete oxidation and environment contamination with chromate. There are methods with external heating to fully oxidize carbon but chromate contamination can not be avoided. Ignition methods are simple but slight overestimatation is expected. Dry combustion methods give precise values of carbon but costly and more careful sample preparation and analysis are required.

Can anyone upload this file

Nelson, D.W., Sommers, L.E., 1996. Total carbon, organic carbon, and

organic matter. In: Sparks, D.L., (Ed.), Methods of Soil Analysis. Part 3.

Chemical Methods, ASA-SSSA, Madison, USA, pp. 961–1010.

Walkley and Black's method is better

Wet digestion by Walkley and Black's methods with cold finger is very good for determination of organic methods especially in Calcareous Soils. But dry combustion is useful for peat and Organic Horizons.

I prefer Loss on ignition but you have to take the below notes as I am a specialist in a such events

Numerous methods measured organic carbon such as titrimetric dichromate redox method, dry combustion-CO2 determination method and wet oxidation-redox titration method . These methods have some difficulties with workers safety and are very time consuming if large number of samples need to be handled.

Walkley and Black method is a long standing measurement of soil organic matter in soils and generates waste containing strong acid and Chromium (Cr), which affect the environment and can lead to blood toxicity for workers.

loss-on-ignition method is a simple and rapid assessment of organic matter in soil. however, it has suffered from some errors :

1- The conversion factor; organic carbon is estimated by applying factor (1.724). This factor is based on the fact that organic matter contains 58% carbon . However, this factor does not provide consistent organic matter estimations for all soils due to different organic carbon contents in soils.

2- Loss-on-ignition can drive off the water bound in clay minerals by volatilizing the soil components such as water in crystalline clay lattices and allophones, hydroxyl group in sequioxides and breakdown of carbonates

method is available on plants journal AL-Maliki and Mugtaba 2018

Interactions between mycorrhizal fungi, tea wastes 2 and algal biomass affecting microbial community, 3 soil structure and alleviating of salinity stress on corn 4 yield (Zea mays L) 5

Al-Maliki Salwan 1*, AL-Masoudi Mugtaba 2

Walkley and Black's method is better for normal agriculture soils. Indignation method will be OK for forest litter soils, compost or any soil which apparently contains high OM.

The professors answered enough

walkley and Black

Certainly, Ignition method is better for soils with high OM.

I think this is a crud methods and its not accurate method for determination . It can be used only with peat and O horizon when organic matter content is more than 10%. it can not used for calcareous soils and Gypsic horizons.

As mentioned by me earlier, the dry combustion method is more precise and accurate but costly and require careful sample preparation and analysis.

the best method of organic matter determination is wet digestion method. It is the Wakley Black methods by using Potassium dichromate> It is more precise and accurate methods for organic matter determination.

I think, the best method of Organic carbon determination ( no organic matter) is the Walkley Black methods. but conversion of organic carbon into organic matter with a constant coefficient (that for surface horizons about 2 and subsurface horizon about 1.5 ) increases its error, because organic carbon content is about 50 to 60 percent of organic matter, than this factor is obtained by dividing 100 by it.

But in dry combustion method there is a weighing error. Therefore, in high percentages of the organic matter, this error can include a small fraction of the total.

I think, Walkley Black methods has two problems, incomplete oxidation ( especially in soils with high organic carbon) and environment contamination with chromate.

Hello Shakir, i have been following Walkey balck method since 4 years sofar i got the best results, hence i recommend this method to follow

Best wishes

Jameer Shaik

Sir it is Walkley Black method using potassium dichromate as an oxidising agent which i am following since i joined in my services.

Dry combustion method is a best method.

Obviously, the dry method, above 750 ºC. There a lot of T.O.C.A.; some of them use IR analyser for the determination of the CO2 emitted. Please select the analyser that use solid samples up to 2,00 g.

2% is Electric furnaces methosd