I have read some different articles including free amino acid analysis of foods in which HPLC, UHPLC or amino acid analyser were used. Which is the best method to analyse these nutritional compounds? Can you especially suggest some references using chromatographic techniques effectively?
Best regards,
Sanem
- The most widely used technique for measuring amino acids in foods is HPLC (not "UPLC" which is a tradename). *Terminology in science is important.
Answer: If you perform a keyword search using any popular search engine (e.g. GOOGLE), then you will find thousands of free articles, papers, application notes and articles to review. For example, keywords such as:"HPLC", "Amino acids" and "Food" will provide you with many examples to review. *As sample prep and method vary, depending on the type of food product, review the articles which match the specific types of food products you are interested in.
- Learning how to initially research a topic is a key skill that should be developed early in your academic studies. The time you put into researching a question will pay back in knowledge and allow you to ask better questions.
Please have an advanced level chromatographer at your school assist you with this project.
BTW: Amino acid analysis by HPLC is one of the more advanced types of methods to run. Years of practical experience and training in online Pre or post-column derivatization is required as are scanning diode-array and/or fluorescence detectors. Successful use of these types of methods require a solid understanding of the fundamental concepts of liquid chromatography plus many advanced level skills. Any work in this area should be carried out with the aid of a highly experienced chromatographer.
Dear William Letter,
Thank you for your answer. I have already read some articles from different countries studied on this analysis. I have learned that some difficulties are available to seperate the amino acids clearly by using HPLC columns. For this reason I would like to learn which method is the best one and if there is a solution about that topic. Thanks again for your interest. I will take account of your feedback and advice.
Kind regards.
You can read aminoacid analysis application of Waters, Agilent, Shimadzu, etc. We performed aminoacid analysis according to applications by using HPLC-DAD system. Sample preparation is so important for this analysis. If you want to analyze only free aminoacid, sample preparation easier than acidic hydrolysis.
Since amino acids are degraded very quickly, it may be better pre-column derivatization. For this, you can get support from the service provider of your instrument. Because you may need some adjustments in the method.
If you have any other questions please don’t hesitate to ask.
best regards
Pinar
Dear Bansi Rameshbhai Pansuriya,
Thank you for your comment.
Kind regards.
You should use HPLC-UV or HPLC-FLD with revers phase column (C18).
Pre-column derivatiztion (phenylisothiocyanate or ortho-phthalaldehyde reagent that you can find their methods in papers) is needed to detect them.
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