Pseudomonas aeruginosa is an oppurtunistic pathogen still we use it as an efficient Plant Growth Promoting Rhizobacteria. How can we check the pathogenecity of P.aeruginosa in laboratory by applying simplest technique
Hello Sakshi,
depends on the mechanisms of pathogenicity that you want to study. Particularly we have been working with Mtb and other respiratory pathogens and the best way to do this is by using a animal model and determine the LD50 or dissemination (if you are comaparing several strains of the same pathogen). Probably also you could use an in vitro infection model (we have used epithelial cells-A549, monocytes and monocytic cell lines-u937, or in vitro differentiated macrophages) these as i said, depends on what mechanisms are you interested in. If you want i can send some references where these methods are described in a detailed manner. Best regards
You may try the C.elegans model that is quite rapid and easy to perofrm
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