I am not an expert on this. When we need to separate mycelium from broth, we use a Buchner funnel with an appropriate Whatman filter, add the broth on top, and rinse the mycelial mat with sterile-distilled water under vacuum until the rinseate is clear (rather than PDA-colored). If I remembered to weigh the Whatman filter before-hand, I can put the filter in a drying oven at 100 C, wait until it dries, weigh it, and have the dry wt biomass of the fungus (by subtracting the weight of the paper).

Thanks a lot, Nina Shishkoff I will use the vacuum filtration method