Butanization and flowering period

At the time of maturity !

Regards

Yes most of the secondary metabolites develop at peak following the physiological maturity of the crop..

Mr. H.N. Nagendra Prasad and Mr. Anoop Kumar Srivastava Thanks for your valuable help.

Secondary metabolites chiefly protect plants. Follow the growth curve of the plant to ascertain the maturity peak. You can extract at that stage. Wishing you the best in your research. Cheers.

This will be dependent on your targeted secondary metabolites. Different secondary metabolites have different concentrations varying over the growing period.

@ Mohamad Abdallah,

I think it depends upon the " targeted secondary metabolites" as rightly said @ Alexis Panny Yin-Sir Chung. Further it may depends upon the plant species on which you are working. I think some volatile compounds may not be available at maturity. So One has to plan according to the secondary metabolites of interest and the material under investigation.

Butanization and flowering period

It depends on what you want to isolate, what class of compounds... And within this class - in what form - aglycones, ethers or glycosylated and other modified forms .. they are usually synthesized during different vegatation periods in various quantities

Thank you all for your valuable help.

Another item that affect on the content and the variety of costituents is the plant type. For example in oregano, higher bioactive compounds are available at maturity but in thyme it is not correct.

How do climacteric and non-climacteric fruits notice such changes in secondary metabolites...

Hi Abdallah, the composition and nature of plant secondary metabolites varies greatly depending on factors such as plant age, genotype, growth medium, light, pH, e.t.c. In this regard, depending on your study objectives you will need to make a number of considerations. For example are you planning to do targeted analysis, profiling or screening? Targeted analysis by HPLC/DAD is only possible for metabolites whose standards are commercially available.

Hi Evans Were , I am looking forward to have a preliminary screening, I am not looking forward to do an analysis in depth. The whole point of this is to do a comparative study of secondary metabolites between different species.

You will hardly find any particular growth stage of any crop , fruits or vegetables , where the concentration of , if not all , most of the secondary metabolites is at maximum.Preliminary study at crop harvest stage will reveal the concentration of at least important metabolites. You have not revealed , which kind of crops , you are using them as test crops...

Anoop Kumar Srivastava

I am working on wild species such prosopis juliflora, prosopis cinerarea, chenopodium album.

It also depends on the purpose of studying these three species.

Let´s say that you want to decide for one point in time to measure the secondary metabolites in these plants.

Therefore, if you are interested in comparing them from the ecological point of view, then it would be more informative to decide for the time when these plants are well established and interacting properly together. Deciding for this time is determined by considering the plant growth stage of the studied species and when they already start interacting. In this case, it is important to consider for sampling shortly before flowering to understand the dynamics of plants interaction (chemically) in the critical stage of plant growth; namely at the vegetative stage.

But, if you are interested in comparing them from the pharmaceutical or commercial point of view or for human consumption (such as Chenopodium album), then it would more informative to estimate the secondary metabolites shortly after flowering (20-50% flowering). In this case, the analysis would provide a more precise estimation of the secondary metabolites and their concentrations when they are used in the market.

It would also be helpful if you clarify the purpose of studying these three species.

Heba Elsalahy Thank you for your valuable help. The purpose of this study is to measure the secondary metabolites of the mentioned species from an ecological perspective in order to understand their intercation dynamics.