Which is the best protocol to pack and activate Amberlite XAD-2 column? Do we have to use vacuum for packing and how to maintain flow rate??

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Jack Silver

I would wash the resin with methanol outside the column, then rinse with water. I'd pack the column in water. The initial wash gets rid of fines and anything left on the column from manufacture.

Christian Alonso Becerra

Hi Ravish.

I think this paper could be useful.

Ravish Godse

Thank you Jack Silver and Christian Alonso Becerra .