As long as you have well-calibrated images, ImageJ is a nice and free software package for this.
http://rsb.info.nih.gov/ij/
To my knowledge, as long as its TIFF image.. Image J should be perfect.
Is your data already rendered into an image (say a topological surface) or do you only have raw data that first needs converted into an image format? ImageJ may have some plugins specific to AFM that could be useful.
Try Gwyddion. http://gwyddion.net/
It has plugins to import AFM format. If your format is not available, you can import TIFF file. It is a freeware.
Here are the resources:
PLUGINS: http://rsbweb.nih.gov/ij/plugins/index.html
TUTORIALS:
http://www.uhnresearch.ca/facilities/wcif/imagej/particle_analysis.htm
http://rsbweb.nih.gov/ij/docs/pdfs/examples.pdf
http://microscopy.berkeley.edu/courses/dib/sections/04IPIII/particles.html
imageJ is open source and can do these kind of things pretty easily
Hi Saeed,
Thank you for your reply
As far as I know nanoscope (in the basic operation) is not enough powerful for nanoparticle size distribution i.e cannot discrimite among two NPs if the NPs are in contact
GR
Hi,
Try this thesis:
http://publications.polymtl.ca/478/1/2010_SimoneBertolazzi.pdf
Start with this sentence and the reference : "The elaboration of the AFM data concerning the nucleation and the growth of tetracene films (described in Section 3.1), was conducted by means of the software WSxM v.5.0, whose functions are reviewed in reference [49]. "
page 75 starting from first paragraph describes how he made the theoretical calculations.
Good luck!
You can try WSxM nanotec software which can be easily downloaded free of cost. It might be helpful .
I am using Image J to prepare particle size histograms from TEM images. There are some helpful functions included under Process and binary. For me this program includes everything i need. When you need more options you can also try FIJI it's an upgrade of Image J.
I also use Image J to obtain information from FE-SEM images. If Your nanostructures distriubtion on the support is regular (e.g. hexagonal) You can obtain also a lot of information from Fast Fourier Transforms from the WSxM programme.
Jordi, take a look at the software listed in the thread "https://www.researchgate.net/post/What_GNU_GPL_microscopy_image_analysis_software_is_available". Any of these software you can use for nanoparticle size estimation from AFM image.
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