Which is the best model for tyrosinase related research, B16 or B16F10 or B16F1?

More Ewirghol Barry's questions See All

Can someone explain the intuition behind, Blue stars are small, but wavelength is big. Red stars are Big but their wavelength is small?

can someone use quantum mechanics to explain the intuition behind this phenomenon?

07 June 2024 9,584 2 View

Does anyone have a working copy of the HFE-Diagram macro developed by Gimenez-Forcada and Sanchez San Roman (2015) that they can share?

Does anyone have a working copy of the HFE-Diagram macro developed by Gimenez-Forcada and Sanchez San Roman (2015) that they can share? The download from the site is not functional. Thanks Barry

27 May 2024 1,908 0 View

How would i calculate the density matrix for the singluarlity at the big bang, and the entanglement entropy?

this is to see if entanglement can replace inflation and/or explain the low entropy at the beginning of the universe.

23 March 2024 4,782 4 View

Is there any evidence that the global decline in food micronutrient levels might be connected to the incidence of medical conditions such as autism?

There has been a global decline in food micronutrient levels since the 1980s. This is related to the way most crops are grown: Monocropping (predominantly corn, soybeans and wheat or rice,...

21 April 2023 1,340 15 View

Can the below mechanism generate purely electric, propellant-less propulsion?

The proposed mechanism is based on BLDC motor technology but utilizes the equal and opposite force imparted to the stator when driving the rotor at speed. Typically, the rotor performs the work,...

01 March 2023 6,258 0 View

Why are air sea fluxes of CO2 many times greater than the surface water net ecosystem production?

It is an empirical fact that CO2 air-sea flux measurements from either eddy covariance or differences in their respective partial pressures with turbulence etc are much greater than the surface...

14 December 2022 5,356 3 View

Can I measure the Ion Leakage/Conductivity of Tobacco plant leaves during Programmed Cell Death at 6hrs and 12hrs?

I need to measure the conductivity of tobacco leaves as they are undergoing PCD at timepoints of 6, 12, 18 and 24hrs. The problem that I have is if I sample the leaves at 6hrs (for example) I...

15 March 2022 9,224 0 View

Help troubleshooting failed phage transduction?

Lysate prep: donor strain is inoculated overnight in LB then diluted 1:100 into 5 mL LB + 0.2% glucose + 5 mM CaCl2, incubated for 1 hour. 100 uL of P1vir was added, then incubated until total...

23 September 2021 8,024 4 View

Help with LDH activity assay?

Is is possible to find the LDH concentration using a solution NAD+, LACATE and CAPS buffer with the LDH sample at 340nm to find the LDH concentration in human blood? what would be the protocol?

26 June 2021 6,790 2 View

Are there studies available that have looked at the minimal clinical important difference (MCID) for STAXI-2?

I am analyzing data from a pre-post study where the STAXI-2 was administered to measure changes in levels of anger. The STAXI-2 has 12 summary measures of 3 domains of anger. The "post" statistics...

09 June 2021 4,599 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

How to restart MD without using checkpint file in GROMACS?

Dear All Gromacs User I would like to restart MD without using checkpint .cpt files? I heard there is a way for acheiveing this. Any input and sugegstions are most welcome. Thanking You Budheswar

29 July 2024 3,084 8 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

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28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Francisco Solano

To study anti-melanogenic effects, you need a line with high content of melanin, in order to see the supposed decrease. Of these lines, the more melanogenic is B16/F10, but anyway, the three lines decrease in pigmentation after several passages. The level of pigmentation depends on so many factors.

Ewirghol Barry

Thank you very much for your answer Dr Francisco, I also read some assays about these researches. Most of those assays indicated that B16F10 is better option in vitro.There are also some passaging problems among those who deal with B16F10 as you mentioned.I will keep in mind you suggestion and work on my new research plan. If you have any suggestion about the passaging , I would love to hear from you .Thank you very much.