photobioreactor consist of layers of tubes used to cultivated Chlorella vulgaris
I wanna suitable a way to clean this tubes
you can prepare a 10% solution of HCl and put tubes on the solution for 24 h and then washing the tube with milli-Q water. If HCl leads to damage to the PBR tubing, you can use the dish sponges. cut a number of sponges a little larger than the diameter of the tubing and then soak them and add it to PBR. it is better to increase the amount of pressure by a pump. the water pressure will force the spange through the tubing system and make it clean. If this method was suitable you need to change the spange every time and repeat it.
Hello!
What we've found to be very effective in cleaning glassware used to grow microalgae is to rub with a sponge first to remove any big chunk of biomass and then soak the glassware in hot solution of sodium carbonate anhydrous (15g per liter) overnight, rinse 3x with distilled water, soak in HCl 2% solution (although not necessary, it is better if you leave it overnight too), and then rinse 3x with distilled water again.
If you manage to remove most of the big pieces of biomass before the washing, you can re-utilize the solutions a few times, if not just prepare new solutions every time you need to clean.
Regards!
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