Which is the basic book for understanding Fluorescence-activated cell sorting ?

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Why I am getting multiple band in SDS-PAGE after protein purification?

I have cloned a gene (750 bp) in pMAL-p5x expression vector. My protein is of size 29 kDa and it is fused with Maltose binding protein (42 kDa). I got 3 bands in SDS-PAGE after the protein...

04 May 2018 2,075 3 View

Agarose gel percentage to see band of 8 kb and 0.1 kb?

I have double digested a vector having size 8 Kb and Insert 100 bp (0.1 Kb). What percent Agarose gel I used use to see both band clearly?

05 June 2016 7,250 16 View

Which E.coli Strain is best for expression of protein from MBP vector?

Can anybody send me detail protocol for expression and purification of protein from MBP vector?

05 June 2016 6,170 4 View

What is the possible reason for getting smear in a gel?

I found smear in gel after double digestion of Plasmid with SfiI and NotI (Lane 4). Single digestion with SfiI works fine (Lane 3). Lane 2 is uncut plasmid. What is the possible reason for getting...

04 May 2016 6,608 8 View

How can I perform gene knock in and knock out simultaneously in a cell line?

I want to insert a GFP gene in a particular locus of a chromosome by replacing already present gene at that site. Which approach will be highly efficient for homologous recombination? Which vector...

04 May 2014 2,079 2 View

How to insert GFP gene in a genomic DNA of U-87 MG (glioblastome cell line)?

Is it possible to insert a GFP gene in gDNA of U87MG by replacing some other gene at that place? If, yes then which GFP vector should I use to transfect in U87MG? Kindly tell me detail procedure...

04 May 2014 3,476 0 View

How can I estimate the copy number of a gene by using SyBR green realtime PCR?

03 April 2014 3,528 13 View

Which is the best freely available software for quantifying cDNA by using Real time PCR?

Method used is ^^CT method

09 October 2012 1,451 23 View

How to reduce star activity in restriction digestion?

I am using BamH1 and EcoR1 for restriction digestion of my gene of 1.3Kb but EcoR1 is also cutting in between the gene. I am getting two fragments of 900bp and 400bp.This is due to presence of...

02 March 2012 8,472 13 View

Cytotoxicity activity of plant extract

Which is the standard cell lines used for checking cytotoxicity activity of medicinal plant extract ?

08 September 2011 1,712 7 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How to show in a scientific paper format the post-hoc test of 4 variables involved, when 3 by 3 variables are significant?

In a study involving 4 variables (species(2x), temperature(2x), nutrients(2x) and light(3x)), 3 by 3 variables, for example, "species", "temperature" and "nutrients" are significant. I end up with...

15 February 2021 8,018 2 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View