Which is the basic book for understanding Fluorescence-activated cell sorting ?

More Mahesh Agarwal's questions See All

Why I am getting multiple band in SDS-PAGE after protein purification?

I have cloned a gene (750 bp) in pMAL-p5x expression vector. My protein is of size 29 kDa and it is fused with Maltose binding protein (42 kDa). I got 3 bands in SDS-PAGE after the protein...

04 May 2018 2,191 3 View

Agarose gel percentage to see band of 8 kb and 0.1 kb?

I have double digested a vector having size 8 Kb and Insert 100 bp (0.1 Kb). What percent Agarose gel I used use to see both band clearly?

05 June 2016 7,335 16 View

Which E.coli Strain is best for expression of protein from MBP vector?

Can anybody send me detail protocol for expression and purification of protein from MBP vector?

05 June 2016 6,246 4 View

What is the possible reason for getting smear in a gel?

I found smear in gel after double digestion of Plasmid with SfiI and NotI (Lane 4). Single digestion with SfiI works fine (Lane 3). Lane 2 is uncut plasmid. What is the possible reason for getting...

04 May 2016 6,690 8 View

How can I perform gene knock in and knock out simultaneously in a cell line?

I want to insert a GFP gene in a particular locus of a chromosome by replacing already present gene at that site. Which approach will be highly efficient for homologous recombination? Which vector...

04 May 2014 2,153 2 View

How to insert GFP gene in a genomic DNA of U-87 MG (glioblastome cell line)?

Is it possible to insert a GFP gene in gDNA of U87MG by replacing some other gene at that place? If, yes then which GFP vector should I use to transfect in U87MG? Kindly tell me detail procedure...

04 May 2014 3,558 0 View

How can I estimate the copy number of a gene by using SyBR green realtime PCR?

03 April 2014 3,616 13 View

Which is the best freely available software for quantifying cDNA by using Real time PCR?

Method used is ^^CT method

09 October 2012 1,550 23 View

How to reduce star activity in restriction digestion?

I am using BamH1 and EcoR1 for restriction digestion of my gene of 1.3Kb but EcoR1 is also cutting in between the gene. I am getting two fragments of 900bp and 400bp.This is due to presence of...

02 March 2012 8,571 13 View

Cytotoxicity activity of plant extract

Which is the standard cell lines used for checking cytotoxicity activity of medicinal plant extract ?

08 September 2011 1,799 7 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

What is this animal ?

In saw this creature during a cable laying operation at 20km off the coast of Timor Leste on the timor sea at a depth of 1200 m.

14 July 2024 4,715 1 View

Please inform me about the International Conference on Plant Biology?

Please inform me about the International Conference on Plant Molecular Biology, or Plant Biology, scheduled for December 2024, January/February, or March 2025.

14 July 2024 3,120 7 View

Changqi Xu

Same question. Let me know if anybody can give some answers. thanks

Ali Hamoudi

Do you have a basic flow cytometry book?

Eva Mezey

Flow Cytometry: First Principles [Paperback]

Alice Longobardi Givan

Mahesh Agarwal

Sir, Do u have soft copy of this book ? If, Yes then kindly attach it here.

Umakant Shinde

You can read it from Wilson and Walker

C. D. Muller

you can fin this at Purdue University by Robinson

just read the Purdue University site, it is important to understand what your are monitoring and how the signal is translated to.

you can download Shapiro's book as well

http://books.google.fr/books?id=JhSyimPKuJwC&lpg=PP1&hl=fr&pg=PA309#v=onepage&q&f=false