11 Questions 8 Answers 0 Followers
Questions related from Mahesh Agarwal
I have cloned a gene (750 bp) in pMAL-p5x expression vector. My protein is of size 29 kDa and it is fused with Maltose binding protein (42 kDa). I got 3 bands in SDS-PAGE after the protein...
05 May 2018 2,147 3 View
I have double digested a vector having size 8 Kb and Insert 100 bp (0.1 Kb). What percent Agarose gel I used use to see both band clearly?
06 June 2016 7,312 16 View
Can anybody send me detail protocol for expression and purification of protein from MBP vector?
06 June 2016 6,218 4 View
I found smear in gel after double digestion of Plasmid with SfiI and NotI (Lane 4). Single digestion with SfiI works fine (Lane 3). Lane 2 is uncut plasmid. What is the possible reason for getting...
05 May 2016 6,671 8 View
I want to insert a GFP gene in a particular locus of a chromosome by replacing already present gene at that site. Which approach will be highly efficient for homologous recombination? Which vector...
05 May 2014 2,134 2 View
Is it possible to insert a GFP gene in gDNA of U87MG by replacing some other gene at that place? If, yes then which GFP vector should I use to transfect in U87MG? Kindly tell me detail procedure...
05 May 2014 3,531 0 View
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04 April 2014 3,590 13 View
Method used is ^^CT method
10 October 2012 1,523 23 View
Especially, the instrument set-up and Data analysis????
10 October 2012 1,086 9 View
I am using BamH1 and EcoR1 for restriction digestion of my gene of 1.3Kb but EcoR1 is also cutting in between the gene. I am getting two fragments of 900bp and 400bp.This is due to presence of...
03 March 2012 8,532 13 View
Which is the standard cell lines used for checking cytotoxicity activity of medicinal plant extract ?
09 September 2011 1,774 7 View
