Using a fixed angle rotor with Sucrose gradient /cushion ultracentirfugation?

I am trying to fractionate virus from plasma into different densities by sucrose gradient but we only have fixed angle rotors... Has anyone tried doing that with a fixed angle? Should I use the...

13 January 2021 8,372 3 View

What are the effective parameters for GPU deep learning card ?

Dear Colleague and experts. I am looking for new laptop with a GPU card for deep learning mission. is there any criteria to make me choose the proper GPU card based on my dataset size and...

27 September 2020 333 6 View

How would ncreasing number of conditions in my current situation affect GLM?

Hi everyone, I have a methodological question to ask. It's my first time performing GLM on SPM12. I'll be measuring BOLD signal over 2x3 ANOVA. Normally, I have 6 conditions, however, if I take...

24 September 2020 9,166 2 View

How can states overcome the catastrophic of COVID 19 and other natural clamitities in this globalised world?

I believe it looks like the 'third world war' is going on where entire world is under lockdown, many have died, many are affected by this Covid 19 catastrophy. Why can not countries findout what...

26 March 2020 8,528 15 View

Can I leave blot in block buffer for 48 hours?

Is it ok ti leave the blot in non fat milk for 48 hours in +4 degrees or is it better to leave it tbs-t for that extra 24hrs?

23 February 2020 7,475 2 View

Should the HCV Core protein fused with His-tag (without linker) be extracted in the insoluble or soluble protein fraction?

I have infected insect cells SF9 with recomb. baculovirus expressing Core.His and another with GFP. I have extracted the soluble proteins after 3 days and did western blot using anti-his,...

10 February 2020 2,359 4 View

Is there any published research on the use of Kember et al.. Reflective thinking questionnaire to measure teachers' reflective thinking?

I am currently working on developing professional development programme to help teachers develop their reflection by engaging in professional development targeting towards improving their critical...

08 June 2019 6,557 2 View

in concrete application Which more benefit adding Nano Carbon tube or Nano cellulose from economic side and performance?

My dear colleagues, in concrete application which more benefit adding Nano Carbon tube or Nano cellulose from economic side and performance?

19 April 2019 4,501 8 View

How using Agilent FieldFox VNA as a signal generator?

I want to take output power from the VNA to feed antennas? But I' m not using VNA as a signal generator.

20 February 2019 5,009 3 View

Why is the antenna designed for a single frequency oscillate at multiple frequencies?

Although we determine the size of the microstrip antenna for a single frequency, why it seems to have a resonance frequency on more than one frequency? Is there an antenna function at each...

13 February 2019 5,064 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

Apart from Nanosilica and CNT's what are the other nanomaterials readily available to use in concrete mixture?

Nanotechnology in concrete materials

01 March 2021 3,379 3 View

Concrete compressive strength databases?

I am looking for datasets similar to this one: http://archive.ics.uci.edu/ml/datasets/Concrete+Compressive+Strength I am looking for concretes with different types of aggregates (Recycled,...

27 February 2021 6,801 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View