I think you can isolate entomopathogenic fungi either from the soil or from infected insect with noticible symptoms of fungal infection

I think it is better to isolate entomopathogenic fungi from infected insects directly, it is easier and need less time.

For entomophagus fungi Isolation should be done from insect gut and for nematophagus fungi isolation should be done from surface of nematode larvae.

However you can screen biocontrol fungi from soil, may be you can invent one or few biocontrol fungi. But it is so time consuming and expensive.

It is easier to isolate entomopathogenic fungi from dead insects than from the soil.

Egg parasitic fungi from the soil for plant parasitic nematode management

Isolation of biocontrol agents from soil is better than isolation from plant parts for managing the diseases.

It is better to isolate the fungi from the plant to use as biocontrol agent

I prefer the isolation from soil concerning soil borne but some antagonists isolats de from plant confirmed their inhibiteur effect against some pathogens.

Thank you Dr Yasmin A. Fergani .

Thank you Dr. Mohammad Abdulhai for answar

Thank you Dr. Anup Kumar Sarkar for answar

Thank yuo Dr. Caesar Kambo

Thank you Dr. Shakti Singh Bhati

Thank you Dr. s.Karpagavalli Karpagavalli

Thank you Dr. L.aura Gasoni

Thank you Dr. Saida Messgo Moumene

I am doing research similar with the topic you questioned.

i prefer to isolate fungi from the plant (especially roots part). root-colonizer fungi is best to compete with soil-borne pathogen. For air-borne pathogen also. because this fungi who live inside plant can activate plant ISR.

for that, you need to examine rheir ability to colonize plant root by inoculation test to the plant

goodluck for your research

Dear wiwiek,

I have purposed the same theme of thesis to my doctorant. I am very interested by scientific collaboration.

Il need the contact of your superviser.

I can give you the contact of my doctorant. Good continuation.

Thank you Dr Wiwiek Harsonowati

Thank you Dr Saida Messgo Moumene

Soil isolates of biocontrol agents preferably from rhizosphere is better than any other part of the plant. Its competitve saprophytic ability (CSA) is more.

In case if plant deseases it id preferbly to isolate pothogen from plant such as Fusarium.if you dont to Know the antagonist effect it id better from soil

Ofcourse it will be from soil

No one is suitable in all situations.

Thank you Dr. s.Karpagavalli Karpagavalli

Thank you Dr. Makhloufi Ahmed

Thank you Dr. Shakti Singh Bhati

Thank you Dr. Harasit Kumar Paul

Hi! I recommend to isolate them from the soil. It's a slow process because you have to test the pathogenicity against insects. In that case, you can use Galleria mellonella larvaes as a hosts. But you can't be full sure that you are goint to isolate only fungi. You can also isolate Entomopathogenic bacterias or nematodes. It's a really good option to use the natural enemies as biological control agents because of their adaptation but if there are several damages in the crop, I prefer use non native in order to reduce the pest population.