Hi, my topic is phytochemical and antioxidant properties of selected species of lamiaceae" i have screened samples by dpph method, now i want to do quantitative analysis which method shoild i use
Quantification of antioxidants involves the estimation of Flavonoids ,Alkaloids ,all phenolics etc .you can try the simple spectrophotometric methods like Follin-cio-caletu for total phenolics ,besides you can go for the HPTLC for exact quantification of the antioxidants using the standards as reference.
Have a look at the attachment also ,hope it will help.
I think attached files can help you to choose best methods. Some researchers believe that just DPPH and ABTS methods are not sufficient to state antioxidant activity in a bioactive compound.
Dear Bhagyashali Karle,
The antioxidant power can be evaluated in vivo or in vitro by applying methods that measure the antioxidant capacity of a molecule or a natural extract. Given the complexity of the oxidation processes and the diverse nature of antioxidants, with both hydrophilic and hydrophobic components, there is not a universal method by which antioxidant activity can be quantitatively measured accurately. Most often, the responses of different and complementary tests must be combined to give an indication of the antioxidant capacity of the test sample.
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