Investigation of heavy metals bioavailability from soil focused on releasable, potentially mobilizable and mobile forms. A single-step extraction procedure was used to isolate the individual forms of metals. The releasable forms were determined in a 2M HNO3 using the present relevant method. The potentially mobilizable forms were extracted with 0.05M EDTA and the mobile forms with 0.1M CaCl2.
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Extraction of soil using EDTA can extract dissolved/soluble and some exchangeable fraction of metals.
EDTA does not target any specific pool of metals in soil/sediments, however, because of its chelating nature it makes soluble complexes with metals and don't let them re-adsorb or precipitate.
In plant sciences, EDTA extractable fraction of metals is generally considered plant available pool of metals.
EDTA can extract soluble , exchangeable , associate with carbonate fractions from soil , so I thank the best method to extract bio-available heavy metal in soil is AB-DTPA. please take a look