Hello!
I plan to check the protective effect of the adoptive transfer of some memory CD8 T-cells against the LCMV infection. So I think to establish LCMV meningitis model and to see whether the mice which have got CD8 T-cells from the LCMV-immune mice will be protected against LCMV meningitis.
In the Current Protocols in Immunology, 2001, M von Herrath and J L Whitton stated that I need to use 1 ml 27G syringes and anesthesia for intracerebral injections (like sedate mice and make a careful injection 5 mm dorsal from the eyes).
Do I need any additional equipment, like stereotaxis, Hamilton syringe or anything else or small insulin syringe is enough?
Do I need to perform LD50 titration or I can just use some sufficient viral titer, let`s say 50-100 pfu/mouse?
I`d be thankful for any tips or advices.
Many thanks!
https://www.msdmanuals.com/home/brain,-spinal-cord,-and-nerve-disorders/meningitis/acute-bacterial-meningitis
https://www.msdmanuals.com/home/brain,-spinal-cord,-and-nerve-disorders/meningitis/acute-bacterial-meningitis
Dear Hiba Riyadh Jameel, thanks for your response. But as far as I can see, it is some kind of a general information about meningitis and as I can see, it's about humans.
Do you have something about the methodology of meningitis induction in mice?
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