use the Pimecrolimus

Class: Skin and Mucous Membrane Agents, Miscellaneous

VA Class: DE900

Chemical Name: (3S,4R,5S,8R,9E,12S,14S,15R,16S,18R,19R,26aS) - 3 - [(1E) - 2 - [(1R,3R,4S) - 4 - Chloro - 3 - methoxycyclohexyl] - 1 - methyl - ethenyl] - 8 - ethyl - 5,6,8,11,12,13,14,15,16,17,18,19,24,26,26a - hexadecahydro - 5,19 - dihydroxy - 14,16 - dimethoxy - 4,10,12,18 - tetramethyl - 15,19 - epoxy - 3H - pyrido[2,1 - c][1,4]oxaazacyclotricosine - 1,17,20,21(4H,23H) - tetrone

Molecular Formula: C43H68ClNO11

CAS Number: 137071-32-0

Brands: Elidel

thanks sir for your reply.....

Which drug can i use as reference for immunomodulatory activity?

Which One enhances the immune response?

You can use cyclophosphamide i.p.; 100-200mg/kg; daily for 4 days. This treatment induce lymphopenia in mice, depleting even Tregs.

Rapamycin would work!

You can use several immunomodulatory agents:

Methotrexate 0.5 mg/kg to 10 mg/kg (0, 24, 48 hour of antigen exposure) and continue antigen exposure weekly or on a biweekly basis. If you have a control group (antigen only) set up along with your immunomodulator group, you will see the difference in antibodies seen against your antigen.

If you want to look at role of immunomodulation in an established immune response setting, look up for bortezomib. Bortezomib is a proteasome inhibitor and targets mature antibody producing plasma cells (which is the final source of antibody production). For this experiment, you can provoke antibodies against your desired antigen and then administer bortezomib (0.75 mg/kg on day 1, 4, 8 and 11) and you will see a drop in antibody titers. you can repeat bortezomib administration to see the further drop in antibodies

Other agents: Rituximab (its a human antiCD20 so you need to find mice specific antiCD20) it targets B cells

Rapamycin as others have mentioned is another agent. Also mycophenolate moefetil, azathioprine, cyclosporine