I would like to get a list of medium which can be used for the isolation and cultivation of Halophiles. Which one can support the pigmentation of Haloarchaea and bacteria?
Dear Sagar
you can use normal nutrient agar and trace metal solution with 40-50 ppm salinity. (preferably you can decide the salinity concentration based on your sampling site). Use low percentage agar for the medium.
Try nutrient agar dissolved in sea salt solution with various salinities.
You can also use sea water to replace salt. Concentrate to various salinities by boiling filtered sea water.
Various media including both rich (eg nutrient agar, LB agar etc) or minimal salt medium may be used, but the important factor is the supplementation with salt (eg NaCl). Add 1M or above concentration of NaCl in the growth medium and you will be able to isolate pigmented halophiles.
my suggestion is Zobell's Marine agar media, It is used in the culture of heterotrophic marine bacteria, the minerals it contains closely mimicking sea-water, but with the additional nutrients, peptone and yeast extract.
you can use TYES media, Mullakhan and Larson media or MS media for cultivation of halophiles
I recommend taking a look at the Halo-handbook. It's a free download online, and a great reference for halophile research! ;)
http://www.haloarchaea.com/resources/halohandbook/
That is dependent on type of halophiles that you want it (slightly or moderately or extremely), you should to search on your type.
Because also halophiles are: Archaea Bacteria, fungi, algae, protozoa.
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