Red pulp macrophages are CD11b-F4/80+CD68+AF+ (see excellent paper by Andrew Mitchell http://www.ncbi.nlm.nih.gov/pubmed/20534703), splenic pool of monocytes will be CD11b+CD115+Ly6C+/-(F4/80lowCD11c-Ly6G-NK1.1-MHC II-) (see Fillip Swirski's Science paper http://www.ncbi.nlm.nih.gov/pubmed/19644120). CD169 and CD209b work great for IFM but I was not able to make them work for flow (interestingly, CD169 works on lymph nodes, but not in the spleen). Please, let me know if you find good flow markers for MZM and MMM or make CD169 or CD209b work.

You may try CD45+ CD11bright F4/80+ Ly6C- for all of them.

They must be all positive for MerTK and CD64

Then more precisely :

Marginal zone Mph in general are SIGN-R1+ and within, metallophilic Mph are CD169+

You may also use some other markers like CD68, MARCO and TIM-4. A very nice paper just came out btw :

http://www.nature.com.ejproxy.a-star.edu.sg/ni/journal/v14/n8/full/ni.2622.html

Good luck!

Thanks a lot for the answer! I have tried MARCO and CD169 in microscopy and they worked fine, but I couldn't manage to do the FACS staining!

For a simple diff try F4/80+, but blocking is an absolute must.

Red pulp macrophages are CD11b-F4/80+CD68+AF+ (see excellent paper by Andrew Mitchell http://www.ncbi.nlm.nih.gov/pubmed/20534703), splenic pool of monocytes will be CD11b+CD115+Ly6C+/-(F4/80lowCD11c-Ly6G-NK1.1-MHC II-) (see Fillip Swirski's Science paper http://www.ncbi.nlm.nih.gov/pubmed/19644120). CD169 and CD209b work great for IFM but I was not able to make them work for flow (interestingly, CD169 works on lymph nodes, but not in the spleen). Please, let me know if you find good flow markers for MZM and MMM or make CD169 or CD209b work.

Moma-1 works well for CD169 in spleens

Hi Anthony, do you mean by FACS or by IHC/IFM? If you have any representative FACS plots for MOMA-1/CD169 could you, please, share it with me? Thank you in advance.