Antisense phenomena is generally absent in bacteria. But u can knock down gene using Sigma targetron kit and is effective for M. tb. Another way is using suicidal vector. Clone gene of interest in suicidal vector and introduce in native strain. If the protein corresponding to gene of interest is prest in bacterial, the bacteria will survive else it will die.
The antisense technology in prokaryotes does not work like in eukaryotes although the production of a complete complementary RNA will decrease the translation of your target RNA; rRNA cannot translate dsRNA.
My advice is to produce a complementary mRNA for your target gene, here an interesting article about that:
Great! Ive read your paper and it's very interesting. I would like to use this vector (pMV361) but I don't know where I could find it. Do you know where can I get it? I'm working in Peru and I would like to introduce this kind of antisense technology applied in M. smegmatis or M. tuberculosis.