I need a single antibody that can be used to label any cell type. Are there any surface proteins expressed in all human cell lines that I could target? If you know an exact antibody, please let me know. Cheers, Alex
Your best bet would be a pan-anti-MHC-I antibody, which would stain every cell type except platelets and erythorocytes. MHC-I is also found inside the cell, so perform the stain prior to permeabilization to ensure it is limited to the surface. A quick google search found this: http://www.scbt.com/datasheet-32235.html. I have never used this antibody before, so I cannot attest to how well it works. Good luck.
What do you want it for? I use the anti-HLA class 1 ABC from Abcam (ab70328) to detect human tumor cells in mice (I work with patient derived xenograft tumors), and it works really well.
Does it need to be an antibody? You could use a DNA stain that is cell permeable (i.e. Syto dyes, DAPI). That will stain every nucleated cell
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